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. 2022 Mar 30;604(7904):167–174. doi: 10.1038/s41586-022-04598-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Genomic tagging of endogenous NOL9 with 3×Flag. b, Experimental design for protein immunoprecipitation from a chromatin fraction. c, Western blots showing fractionation of HEK 293FT cells. d, Silver-stained gel of Flag immunoprecipitations from wild-type and Flag–NOL9-expressing HEK 293FT cells. e, Volcano plot displaying results of tandem-mass-tag mass spectrometry of proteins enriched in Flag immunoprecipitations from Flag–NOL9-expressing cells relative to untagged cells from two independent experiments. P values calculated by two-sided t-test. Subunits of the rixosome, PRC1, PCR2 and H3K9me3-associated HP1 proteins are highlighted. f, Immunoprecipitations (IP) showing the association of RING1B and EZH2 with Flag–NOL9 in HEK 293FT cells with or without benzonase treatment. IB, immunoblot. g, Immunoprecipitations showing the association of RING1B with rixosome subunits PELP1, TEX10, SENP3 and WDR18 in HEK 293FT cells with or without benzonase treatment. h, Volcano plot displaying mass spectrometry results of proteins enriched in Flag immunoprecipitations from cells expressing Flag–PHC2 relative to untagged cells from two independent experiments. P values calculated by two-sided t-test. Subunits of the rixosome, PRC1, PCR2 and selected other proteins are highlighted.