Extended Data Fig. 8. Colocalization of the rixosome with H2AK119ub1 in human ES and HeLa cells.
a, Matrix depicting Spearman correlation coefficients between ChIP-seq datasets calculated using read counts summed +/−2 kb for all annotated gene TSSs (hg19) in human embryonic stem (ES) cells. b, Heatmap representations of ChIP-seq of TEX10 and histone modifications (H2AK119ub1, H3K27me3, H3K9me3, H3K79me3, and H3K4me3,) in human ES cells. Rank order is from most to least TEX10 signal. Enrichment levels (log2) were normalized with Reads Per Genome Coverage. Read counts per gene were summed in 50-nt bins. c, Matrix depicting Spearman correlation coefficients between ChIP-seq datasets calculated using read counts summed +/−2 kb for all annotated gene TSSs (hg19) in HeLa cells. d, Heatmap representations of ChIP-seq of MDN1, H2AK119ub1, and H3K27me3 in HeLa cells. Rank order is from most to least TEX10 signal. Enrichment levels (log2) were normalized with Reads Per Genome Coverage. Read counts per gene were summed in 50-nt bins. e, Genomic snapshots of ChIP-seq reads at Polycomb target HOXA cluster in human ES (top) and HeLa (bottom) cells for the indicated rixosome subunits or Polycomb histone modifications. Enrichment levels (log2) were normalized with Reads Per Genome Coverage. f, Venn diagram showing overlap between TEX10- and H2AK119ub1-enriched TSSs in human ES cells. Hypergeometric probability p values, 2.9e-4262 g, Venn diagram showing overlap between MDN1- and H2AK119ub1-enriched TSSs in HeLa cells. Hypergeometric probability p values, 8.9e-2935.