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. 2022 Mar 30;604(7904):167–174. doi: 10.1038/s41586-022-04598-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a, Immunoblot showing protein levels in control siControl (siCtrl), siNOL9, siNOL9+NOL9 wild type expressing plasmid, and siNOL9+ NOL9-K312A expressing plasmids. Actin served as a loading control. b, Immunoblot showing protein levels in control siControl (siCtrl), siLAS1L, siLAS1L+LAS1L wild type expressing plasmid, and siLAS1L+ LAS1L-R155A/H160A (LAS1l-2A) expressing plasmids. GAPDH served as a loading control. c, Immunoblot showing protein levels in control siControl (siCtrl), siXRN2-1 (siRNA 1), siXRN2-2 (siRNA 2), siXRN2-1+XRN2 wild type expressing plasmid, and siXRN2-1+ XRN2 E203G expressing plasmids. GAPDH served as a loading control. d, RT-qPCR analysis of RNA levels of H2B-CTRN in the indicated siRNA-treated and NOL9-rescued HEK293FT cells after 21 days of Doxcyline treatment. RNA expression levels were normalized to ACTB, and every knockdown was normalized to siCtrl. Error bars represent standard deviations for three biological replicates. Data are presented as mean values +/− SEM.

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