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. 2022 Mar 30;604(7904):167–174. doi: 10.1038/s41586-022-04598-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Diagrams showing the composition of the rixosome and Polycomb complexes. b, Silver-stained gel of Flag immunoprecipitations from untagged (no tag) and Flag-WDR18 HEK293FT cells. c, Volcano plot of TMT mass spectrometry results showing log2-fold changes in proteins enrichment in Flag immunoprecipitations from Flag-WDR18 versus untagged cells from two independent experiments. p values calculated by two-sided student’s t test. Rixosome, PRC1, PCR2, and HP1 proteins are highlighted in green, blue, magenta, and yellow, respectively. d, Immunoprecipitations (IP) showing the association of RING1B with rixosome subunits PELP1, TEX10, SENP3 and Flag-WDR18 in HEK293FT cells. e, Volcano plot of mass spectrometry results showing log2-fold changes in proteins enrichment in Flag immunoprecipitations from Flag-CBX4 versus untagged cells from two independent experiments. p values calculated by two-sided student’s t test. Rixosome and PRC1 subunits are highlighted in green and blue, respectively. f, Yeast two-hybrid assays. Yeast cells transformed with the indicated plasmids were plated onto double dropout (Non-selective) (SC-Trp, -Leu) or quadruple dropout (Selective)(SC-Trp, -Leu, -His, -Ade) medium. AD, Activation Domain; BD, Binding Domain. g, Diagram of the RING1B protein and the binding activities of its indicated truncations. CC1 and CC2, coiled-coil domains 1 and 2. h, i, Pull-down assays using bacterially expressed and purified TEX10 proteins and the indicated bead-immobilized GST or GST-fusion RING1B WT or mutant proteins. TEX10 detected by immunoblotting using an anti-TEX10 antibody. GST-tagged proteins were stained with Coomassie. The assays were performed three time independently with similar results.