Extended Data Fig. 1. NHS esters enable stable, non-toxic, labeling of organ surface ECM.
a-c, Representative immunohistological stainings for NHS labelling, cell death (Caspase3) and immune cell (CD45) amount in control and NHS-labeled organ surfaces. Peritoneal closure and liver electroporation acted as positive control for Caspase3 stainings. n = six biological replicates. (C57BL/6 J WT mice) and three independent experiments. d, Representative images from liver and peritoneal surfaces of animals locallylabelled with NHS-FITC or NHS-AF647 24 hours post injury. n = four biological replicates. Scale bar: 2000 µm. Fluorometer based analysis of FITC signals detected in wounds 24 hours after injury quantified signal to autofluorescence ratio. n = four biological replicates and three independent experiments. Data represented are mean ± SD. Two-tailed Mann–Whitney; * P < 0.05. e, Representative multiphoton and immunohistological images of a liver wound 2 weeks after injury. n = six biological replicates. Scale bar: Multiphoton: 50 µm; Histology: 50 µm. Two-tailed Mann–Whitney; * P < 0.05; ns= not significant. n = six biological replicates. (C57BL/6 J WT mice) and four independent experiments.