FIGURE 5.
UM‐SCC‐108 is sensitive to combined epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) inhibition in vivo. Two million UM‐SCC‐108 cells were injected into each flank of athymic nude mice. Mice were treated with vehicle, 150 mg/kg gefitinib, 30 mg/kg BGJ398, or combination. (A) Following a single 6‐h treatment, mice were humanely euthanized and tumors were harvested. Western blot analysis was performed for indicated proteins, and HSP90 was used as a loading control. Over the course of 3 weeks of treatment (five treatments/week), (B) tumor volume (mean ±95% CI) were recorded for n = 7–8 based on previous experience with similar xenografts 32 , 35 [Color figure can be viewed at wileyonlinelibrary.com]