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. Author manuscript; available in PMC: 2023 Apr 5.
Published in final edited form as: Cell Metab. 2022 Mar 16;34(4):549–563.e8. doi: 10.1016/j.cmet.2022.02.012

Figure 4. Deletion of Ptprd renders AgRP neurons unresponsive to asprosin.

Figure 4.

(a) Schematic figure showing unilateral stereotactic injection of virus (AAV) containing Ptprd sgRNA with AAV expressing mCherry (control) or Cas9 (AgRPPtprd-KO) in the arcuate (ARC) nucleus of adult AgRP-cre male mice

(b-d) Representative action potential firing traces, baseline neuronal firing rate and membrane potential of AgRP neurons from AgRP-cre mice subjected to overnight fasting or ad libitum feeding (control + fed: n = 28; control + fasted: n = 31; Ptprd KO + fed: n = 31; Ptprd KO + fasted: n = 28) after unilateral stereotactic injection of Ptprd sgRNA + mCherry expressing AAVs on one side (control side), and Ptprd sgRNA + Cas9 expressing AAVs in the other side (AgRPPtprd-KO; KO side) ARC of hypothalamus

(e-g) Representative action potential firing traces, neuronal firing rate and membrane potential of AgRP neurons from AgRP-Cre mice, incubated with recombinant GFP or asprosin for 2 hours (Control+GFP: n = 13, Control+Asprosin: n = 13, Ptprd KO+GFP: n = 13, Ptprd KO+Asprosin: n = 11) after unilateral stereotactic injection of Ptprd sgRNA + mCherry expressing AAVs on one side (control side), and Ptprd sgRNA + Cas9 expressing AAVs in the other side (AgRPPtprd-KO; KO side) of ARC of the hypothalamus.

*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001; by two-tailed Student’s t-test. Data are represented as mean ± SEM.