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Phage modification via chlorooxime-cysteine conjugation. a) Illustration of phage modification with FC4 and FC5, and the streptavidin pulldown assay to quantify labeling efficiency. b) Results of streptavidin pull-down assay showing FC4/5 elicited efficient phage modification at 10 μM within 30–60 min. The positive control molecule, Biotin-IA, was used at 1 mM for 2 hr to give a similar percentage of labeling.
