Primary neurons were treated with AMPA, vehicle + AMPA, RVX-208 + AMPA, or JQ1 + AMPA for 2 hours. The expression of (A) Bdnf, (B) Arc, (C) Nr4a1, (D) c-fos, and (E) Gria1 was measured via RT-qPCR. Horizontal dotted line indicates baseline expression of untreated neurons. *P < 0.05 and ****P < 0.0001 indicate a significant difference compared to AMPA or Vehicle + AMPA using Bonferroni post hoc test. Data are mean ±SEM, n = 6 per treatment.