TABLE 2.
Oligodeoxynucleotides used for gene mapping
| Primer | Nucleotide sequence | Positiona | Product size (bp) |
|---|---|---|---|
| RD1 | 5′-CCGTTTAACCCGCTGGAA | 304–321 | 1,269 |
| SD2 | 5′-CGAATGGTGGTATTCTC | 1556–1572 | |
| SD1 | 5′-CTATCATGATCGGGATG | 865–881 | 1,346 |
| YD2 | 5′-CTCTGGAACTGAGGGTA | 2194–2210 | |
| YD1 | 5′-GATTCGTCAACCGCATG | 2433–2449 | 746 |
| HD2 | 5′-ATCCAGCATTGCTTTCTG | 3161–3178 | |
| HD1 | 5′-CGTAAGCCATAAAGCGGA | 3204–3221 | 1,783 |
| DP2 | 5′-AAACCGGCTGCTGTCATCATG | 4966–4986 | |
| DP1 | 5′-AATCACAAAATCCGGCG | 4132–4148 | 1,499 |
| XD2 | 5′-TATGTATCCGGGTATGG | 5614–5630 |
a
Nucleotide numbering begins at the first base of the vanRD gene. Oligodeoxynucleotides were synthesized in the Unité de Chimie Organique, Institut Pasteur, Paris, France.