TABLE 3.
d,d-Peptidase activities in extracts from E. faecium BM4416
| Concn of vancomycin (μg/ml) | d,d-Dipeptidase activitya (nmol min−1 mg−1)b |
d,d-Carboxypeptidase activityc (nmol min−1 mg−1)b
|
|
|---|---|---|---|
| Without penicillin G | With penicillin G (10 mM) | ||
| 0 | 10.1 ± 2.1 | 14.9 ± 2.6 | <10−1 |
| 8 | 10.4 ± 1.7 | 13 ± 0.5 | <10−1 |
| 16 | 10.8 ± 1.6 | 12.4 ± 1.8 | <10−1 |
| 64 | 13.8 ± 4 | 13.1 ± 1.3 | <10−1 |
a
The activity was measured in the 100,000 × g supernatant of lysed bacteria.
b
Values are the means ± standard deviations for a minimum of three independent experiments.
c
The activity was measured in the resuspended pellet fraction after centrifugation of lysed bacteria at 100,000 × g for 45 min.