Fig. 5.

Differential recruitments of transcription factors such as AhR, Arnt, Nrf2, and MafK to ARE and DRE sites in WT and Jdp2^−/− MEFs. a Schematic representation of the mouse AhR promoter and the position of cis elements, such as ARE1, ARE2, DRE1, and DRE2/3, which were detected in the ChIP assay. a–d; f–h The regions that were amplified by PCR with the specific corresponding primers (ARE1, ARE2 and DRE1) and (e, i) with the primers that contained the DRE 2 and 3 cis elements were indicated in WT MEFs. The ChIP-qPCR analyses were performed using chromatin extracts from WT (b–e) and Jdp2^−/− MEFs (f–i) with the indicated antibodies and normal IgG (as a negative control). The probes of ARE1 (b, f), ARE2 (c, g), DRE1 (d, h), and DRE2/3 (e, i) are shown in the presence of 0.1% DMSO, respectively. The values in the absence of DMSO are detailed in Fig. S6. Values represent mean ± SEM (n = 5). Statistical analysis was performed by one-way ANOVA with Tukey’s test (** P < 0.01)