Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 6;13:1853. doi: 10.1038/s41467-022-29479-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Mitochondria isolated from 293T DELE1^HA OMA1 knockout (ΔOMA1) cells treated with DMSO or CCCP for 4 h were processed as in Fig. 2l. b, c HeLa DELE1^HA cells (b) and HeLa cells stably expressing DELE1-HA (c) were pretreated with CHX to deplete newly synthesized DELE1 protein, followed by co-treatment of CHX with CCCP for the indicated times and analysis of DELE1 protein by immunoblotting. CCCP only and simultaneous CHX + CCCP treatment without CHX pretreatment serve as controls. d Schematic comparing wild-type DELE1 and chimeric DELE1 proteins carrying a heterologous MTS and their associated protease cleavage sites, used in the subsequent panels. e 293T DELE1 knockout cells stably expressing the indicated DELE1 variants were treated with CCCP or OM for 6 h. Processing of the DELE1 proteins was analyzed by immunoblotting. f 293T DELE1 knockout cells were transiently transfected with the specified DELE1 chimeras, treated for 4 h as indicated and analyzed by confocal microscopy. Scale bars, 20 μm. Nuclei (DAPI, blue), mitochondria (MitoTrackerRed, pink), DELE1 (HA, green). g Subcellular fractionation reveals the localization of different DELE1 species to mitochondria or cytosol upon CCCP or OM treatment in 293T DELE1 knockout cells transiently transfected with the indicated variants.