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. 2022 Apr 6;5:326. doi: 10.1038/s42003-022-03251-w

Fig. 6. GSTM2 KO induced hepatic fat accumulation was suppressed by GS-4997 in vivo.

Fig. 6

a The GSTM2 KO mice were fed with chow diet and treated with GS-4997 or DMSO at day 1 and 3, then the liver tissues were collected for further analysis. b GSTM2 KO mice were treated with GS-4997 (ASK-1 inhibitor) by intraperitoneal injection. Hepatic fat was detected by HE and ORO staining. c TG content examination of WT mice fed MCDD with GS-4997 or DMSO treatment. d WB detection of expression level of p-ASK1 and LC3 in GSTM2 KO mice liver. e Investigation of protein levels of ASK1 signalling pathway in GSTM2 KO mice with GS-4997 or control, DMSO treatment by Western blot. f Fray value analysis of (e) by ImageJ software. g The WT and GSTM2 KO mice were fed with MCD diet for one month. The mice were treated with GS-4997 or DMSO at day 14, 21 and 28, and then the liver tissues were collected for further Analysis. h GSTM2 KO mice were fed MCDD and treated with GS-4997 or DMSO by intraperitoneal injection. Then hepatic fat and fibrosis level were detected by HE, ORO and Masson staining. i TG content examination of GSTM2 KO mice fed MCDD with GS-4997 or DMSO treatment. bar, 50 μm.