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. 2022 Apr 6;12:5809. doi: 10.1038/s41598-022-09697-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AKR1C3 immunoreactivity of four antibodies on control cell lines. Immunoreactivity of Thermo Fisher Scientific (Clone#PA5-23667), rabbit monoclonal Abcam [EPR16726] (ab209899) and Sigma/Millipore Anti-AKR1C3 antibody, mouse monoclonal, clone NP6.G6.A6, on different control cell lines. HCT116 is a human colorectal cancer cell line. HCT116-AKR1C3 is HCT116 engineered overexpress AKR1C3. Nalm6 is a Pre-B cell ALL cell line with very low endogenous AKR1C3 expression. TF1 is an erythroleukemia cell line with high endogenous AKR1C3 expression.