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. 2022 Apr 6;13:1875. doi: 10.1038/s41467-022-29481-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Time-lapse images of HeLa cells co-expressing mKate-tagged EqtSM (green) and eGFP-tagged CHMP4B (magenta) and treated with 1 mM LLOMe for the indicated time. White arrows mark EqtSM-positive puncta that gradually accumulate CHMP4B. Scale bar, 5 μm. b Time-course plotting EqtSM- and CHMP4B-positive puncta normalized to maximum in cells treated as in (a). Data are means ± SD. n = 5 cells. c Fluorescence images of wild-type (WT) and SMS-KO HeLa cells treated with 1 mM LLOMe for the indicated time, fixed and then stained with DAPI (blue) and antibodies against CHMP4B (green) and actin (magenta). Scale bar, 10 μm. d Time-course plotting normalized CHMP4B intensity in cells treated as in (c). For each violin plot, the middle line denotes the median, and the top and bottom lines indicate the 75th and 25th percentile. In chronological order: n = 40 (WT), 40 (SMS-KO), 60 (WT), 60 (SMS-KO), 60 (WT), 60 (SMS-KO), 92 (WT), 70 (SMS-KO), 80 (WT), 79 (SMS-KO), 81 (WT), 87 (SMS-KO), 82 (WT), 78 (SMS-KO), 96 (WT), 101 (SMS-KO), 70 (WT) and 70 cells (SMS-KO) over two independent experiments. P values were calculated by unpaired two-tailed t test.