Table 4.
Combined analysis of NKG2C (KLRC2) and FcγRIIIA (FCGR3A) genotypes – cohort 1 (BORTEJECT).
| Number of risk genotypes | ||||
|---|---|---|---|---|
| (KLRC2 wt/wt and/or FCGR3A-V/V158 or -V/F158) | ||||
| Parameters | Two risk genotypes | One risk genotype | No risk genotype | P value |
| n=47 | n=31 | n=7 | ||
| DSA characteristics | ||||
| MFI of the immunodominant DSA, median (IQR) | 3,470 (1,753–9,602) | 2,239 (1,360–4,837) | 1,508 (1,087–1,946) | 0.071 |
| Biopsy results a,b | ||||
| Banff ABMR, n (%) | 33 (70) | 14 (45) | 2 (29) | 0.024 |
| g+ptc scorec, median (IQR) | 2 (0–4) | 0 (0–2) | 0 (0–1) | 0.006 |
| MMDx results | ||||
| ABMR archetype, n (%) | 27 (60) | 13 (43) | 1 (14) | 0.052 |
| ABMR score c , median (IQR) | 0.43 (0.14–0.72) | 0.23 (0.08–0.60) | 0.11 (0.10–0.22) | 0.043 |
| NK cell burden-associated PBT (NKB), median (IQR) | 1.01 (0.50–1.35) | 0.61 (0.43–1.22) | 0.43 (0.32–0.69) | 0.074 |
| IFN-γ-associated PBT (GRIT1), median (IQR) | 0.79 (0.46–0.98) | 0.57 (0.29–0.94) | 0.28 (-0.10–0.44) | 0.012 |
ABMR, antibody-mediated rejection; DSA, donor-specific antibody; g, glomerulitis; IQR, interquartile range; MFI, mean fluorescence intensity; PBT, pathogenesis-based transcripts; ptc, peritubular capillaritis.
a
Morphologic lesions were scored according to the Banff 2017 classification of renal pathology.
b Gene expression analysis was performed in 83 of the 86 study patients.
c
For 2 and 4 recipients, biopsy material was not sufficient for ptc, and g scoring, respectively.