Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 6;13:1864. doi: 10.1038/s41467-022-29239-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Assay for Ni(II)-dependent switching. b Ni(II)-dependent switching as measured by the relative rate of Z-Ala-Pro-pNA hydrolysis upon addition of Ni(II) (green points) or EDTA (black points); average rates are shown as green or black dashed lines, respectively. Data points are averages of triplicate reactions and error bars represent standard deviations. c Intact protein ESI-MS data for POP_167/517 in the presence of excess EDTA (black) or Ni(II) (green). Raw MS data (left) was deconvoluted (right) using m/z windows of either 700–900 or 1100–1500 for the Ni(II)-treated protein. d UV–Vis (left) and CD (right) spectra for apo (black) or Fe(II)-treated (pink) POP_167/517 showing expected MLCT transition and intraligand charge transfer, respectively, in the presence of Fe(II). e A representative structure of Ni(II)-bound POP_167/517 from MD simulation shows coordination of two BpyAla residues and two waters to the Ni(II) center. Source data are provided as a Source Data file.