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. 2022 Apr 6;13:1857. doi: 10.1038/s41467-022-29501-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Tomogram slice-through of: a of yeast S. cerevisiae with several ribosomes (orange), potential mitochondria (yellow), and the nuclear envelope (light blue) highlighted; b E. coli BL21 (DE3) + Leviviridae PP7-PP7 virus capsid proteins with several virus capsids (red-orange), ribosomes (orange), and neighboring cell-cell membranes (green) highlighted; c HEK 293 S GnTI- with several transmembrane proteins (blue), ribosomes (orange), and actin networks (purple) highlighted; and d porcine thyroglobulin (ThG) single particle waffle lamellae with several orientational views highlighted (red). Scale bars are 200 nm. Each slice shown is about 8 Å thick. n > 25 a, 35 b, 5 c, and 10 d independent tomograms.