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. 2022 Mar 24;13:845930. doi: 10.3389/fphar.2022.845930

FIGURE 3.

FIGURE 3

Neuronal Toll-like receptors (TLRs) are induced with LPS-activated HMC and downstream signaling. (A) SH-SY5Y cell lysates were collected after coculture with 0.5 and 1 μg/ml LPS-activated HMC for 24 h, and the expressions of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) in SH-SY5Y were analyzed by western blot. Actin was used as a loading control (N = 4). All bars stand for mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way multiple comparison ANOVA. (B,C) SH-SY5Y cells cocultured with 0.5 and 1 μg/ml LPS-activated HMC for 24 h, and cocultured SH-SY5Y cells were fixed in paraformaldehyde and stained with (B) TLR2 (red) and (C) TLR4 (red) (N = 3). Intensity was measured using corrected total cell fluorescence (CTCF). Scale bar represents 10 μm. All bars stand for mean ± SEM; ***p < 0.001 by one-way multiple comparison ANOVA. (D) Expression levels of p-JNK, p-p38, and p-ERK were analyzed by western blot. Actin was used as a loading control (N = 4). All bars stand for mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way multiple comparison ANOVA.