Figure 2.

PUB-I CDR3β-sequences can be found in different individuals with small nucleotide-differences as a result of convergent recombination. The library of virus-specific CDR3β AA-sequences contained 131 sequences that were found in multiple different individuals (≥2). The nucleotide-sequences of these CDR3β AA-sequences were analyzed to investigate differences and similarities. (A, B) Shown are two representative examples of the frequencies of the PUB-I CDR3β AA-sequences CASQDRLTGGYTF and CASSSLNTEAFF, that were specific for HLA-B*07:02-restricted EBV-EBNA3A^RPP and HLA-B*08:01-restricted EBV-BZLF1^RAK, respectively. (C, D) Shown are the nucleotide-sequences of the CDR3β AA-sequences CASSQDRLTGGYTF and CASSSLNTEAFF that were shared by different individuals. Underlined nucleotides represent differences between the different individuals. Nucleotides in red represent differences to the consensus sequence. Nucleotide-sequences in blue and green represent perfect alignment with the germline sequences of the TRBV-gene and TRBJ-gene, respectively. AA, amino-acids; nt, nucleotides.