Figure 3.

PUB-I and PUB-HS CMV, EBV and AdV-specific CDR3β AA-sequences are common in different individuals and of high frequencies within the T-cell specificities. (A) Nine-teen different specificities contained PUB-I CDR3β-sequences, shared by at least 2 individuals. Six specificities contained 2 or 3 different (with different TRBV and/or TRBJ-genes) CDR3β AA-sequences that were found frequently in different individuals. The total numbers of different T-cell populations (different donors) that contained the PUB-I or PUB-HS CDR3β AA-sequences are indicated at the inner-side of the y-axis. The occurrence, shown as percentage among healthy individuals, is shown per CDR3β AA-sequence. PUB-I sequences are shown in grey. Individuals containing PUB-HS sequences with 1, 2 or 3 AA-differences (shown in light-dark orange) compared to the PUB-I sequence were stacked on top of the individuals where the PUB-I sequence was already identified. (B) Shown is the sum of frequencies of the PUB-I and PUB-HS (1, 2 and 3 AA-differences) CDR3β AA-sequences per individual. Each dot is one individual, and the red-lines represent the medians with interquartile ranges. AA, amino-acids; nt, nucleotides; Δ, difference(s).