. 2022 Apr 2;18:20–24. doi: 10.1016/j.ijppaw.2022.03.012

Table 2.

Comparison of real-time PCR, conventional PCR, and DiroCHEK® Heartworm Antigen Test Kit results from Dirofilaria immitis positive coyotes in Texas, USA. Optical density (O.D.) values (DiroCHEK®) are included.

Sample	Real-time PCR^a	Conventional PCR^a^,^b	DiroCHEK®	O.D. readings
Sample	Real-time PCR^a	Conventional PCR^a^,^b	DiroCHEK®	Sample	- control	+ control
10	+	-	+	0.205	0.046	0.454
15	+	–	–	0.052	0.046	0.410
24	+	+	–	0.056	0.069	0.753
26	+	–	–	0.048	0.046	0.454
27	+	–	–	0.049	0.069	0.753
28	-	-	+	0.066^c	0.069	0.753
39	-	-	+	0.373	0.046	0.410
49	+	+	+	0.700	0.047	0.625
73	+	+	+	0.199	0.047	0.625
84	+	+	–	0.057	0.045	0.529
85	-	–	+	0.281	0.045	0.529
101	-	–	+	0.420	0.048	0.493

PCR amplification of the cytochrome oxidase c subunit I gene.

PCR amplification of the NADH dehydrogenase subunit 5 gene.

Antigen was detected by visual color change but did not exceed the cutoff for positive on the spectrophotometric assay used.