TABLE 4.
The characterized genes and the most probable CYP450 genes involved in camptothecin biosynthesis.
| Description | Abbreviation |
C. acuminata gene ID |
GenBank number of the top-hit gene | Identity (%) | Coverage (%) | Module |
| Anthranilate synthase | ASA1 | Cac_g008782 | AAU84988.1 | 100.00 | 99 | Tan |
| ASA2 | Cac_g018456 | AAU84989.1 | 96.72 | 99 | Tan | |
| Tryptophan synthase | TSB | Cac_g001032 | AAB97087.1 | 100.00 | 99 | Green |
| Tryptophan decarboxylase | TDC1 | Cac_g018974 | AAB39708.1 | 97.41 | 99 | Green-yellow |
| TDC2 | Cac_g023139 | AAB39709.1 | 98.19 | 99 | Black | |
| 1-deoxy-D-xylulose-5-phosphate synthase | DXS | Cac_g024944 | DQ848672.1 | 77.38 | 91 | Tan |
| 1-deoxy-D-xylulose-5-phosphate reductoisomerase | DXR | Cac_g016318 | ABC86579.1 | 88.16 | 99 | Magenta |
| 4-diphosphocytidyl-methylerythritol 2-phosphate synthase | CMS | Cac_g018722 | FJ177510.1 | 77.15 | 81 | Brown |
| 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | CMK | Cac_g021688 | DQ848671.1 | 76.90 | 98 | Turquoise |
| 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase | MECS | Cac_g008169 | AF250236.1 | 72.65 | 99 | Green-yellow |
| 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase | HDS | Cac_g022763 | AY184810.2 | 88.65 | 99 | Red |
| 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase | HDR | Cac_g014659 | DQ864495.1 | 99.35 | 99 | Midnight-bule |
| Isopentenyl diphosphate isomerase | IPI1 | Cac_g008847 | AAB94132.1 | 99.15 | 88 | Grey60 |
| IPI2 | Cac_g027591 | AAB94133.1 | 100.00 | 99 | Turquoise | |
| Geranyl diphosphate synthase | GPPS | Cac_g026508 | ATZ76916.1 | 80.57 | 68 | Grey60 |
| Geraniol synthase | GES | Cac_g014037 | ALL56347.1 | 100.00 | 99 | Green |
| Hydroxygeraniol oxidoreductase | GOR | Cac_g027560 | KF302069.1 | 72.18 | 97 | Magenta |
| Geraniol-10-hydroxylase | G10H | Cac_g027852 | JF508378.1 | 94.61 | 96 | Turquoise |
| 10-hydroxygeraniol oxidoreductase | 10HGO | Cac_g005530 | AY342355.1 | 100 | 99 | Turquoise |
| Iridoid synthase | IS | Cac_g006027 | AON76722.1 | 100.00 | 99 | Green |
| Iridoid oxidase | IO | Cac_g032709 | KF302066.1 | 78.44 | 96 | Green |
| UDP-glucose iridoid glucosyltransferase | 7-DLGT | Cac_g008744 | AB733667.1 | 77.11 | 93 | Yellow |
| Loganic acid methyltransferase | LAMT | Cac_g005179 | KF415116.1 | 53.91 | 96 | Magenta |
| CYP72A565 | 7-DLH/CaSLAS1 | Cac_g017137 | QDC27812.1 | 100.00 | 99 | Black |
| CYP72A610 | 7-DLH/CaSLAS2 | Cac_g012666 | QDC27813.1 | 99.62 | 99 | Tan |
| NADPH-cytochrome P450 reductase | CaCPR1 | Cac_g008486 | AJW67229.1 | 99.15 | 99 | Turquoise |
| CaCPR2 | Cac_g008487 | KP162177.1 | 98.31 | 99 | Turquoise | |
| Strictosidine beta-D-glucosidase | CaSGD | Cac_g024440 | AES93119.1 | 68.36 | 95 | Purple |
| 10-Hydroxycamptothecin O-methyltransferase | Ca10OMT | Cac_g014785 | MG996006.1 | 92.90 | 99 | Yellow |
| Strictosidine synthase | CaSTR1 | Cac_g030447 | QWX38542.1 | 100.00 | 99 | Green |
| CaSTR2 | Cac_g030435 | QWX38543.1 | 100.00 | 99 | Black | |
| CaSTR3 | Cac_g030441 | QWX38544.1 | 100.00 | 99 | Green | |
| Oxidase | CYP71 | Cac_g012391 | PWA73981.1 | 70.00 | 99 | Black |
| Oxidase | CYP71 | Cac_g012484 | OVA18686.1 | 66.03 | 93 | Black |
| Oxidase | CYP71 | Cac_g015559 | RVW70431.1 | 67.83 | 96 | Green |
| Oxidase | CYP71 | Cac_g015564 | RVW70431.1 | 66.30 | 89 | Green |
| Oxidase | CYP72 | Cac_g023088 | BAX04012.1 | 63.53 | 98 | Black |
| Oxidase | CYP72 | Cac_g031955 | BAX04010.1 | 71.94 | 95 | Black |
| Oxidase | CYP72 | Cac_g031956 | BAX04010.1 | 70.14 | 95 | Black |
| Oxidase | CYP81 | Cac_g006015 | QNS29972.1 | 70.53 | 99 | Black |
| Oxidase | CYP81 | Cac_g032235 | QNS29988.1 | 59.26 | 98 | Green |
| Oxidase | CYP81 | Cac_g032228 | OMO70595.1 | 71.43 | 66 | Green |
| Oxidase | CYP81 | Cac_g032236 | QNS29988.1 | 59.49 | 98 | Black |
| Oxidase | CYP81 | Cac_g032246 | QNS29936.1 | 60.95 | 93 | Green |