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Forks with unstructured loading strands are unwound at the fastest rate by Pif1. All time course experiments shown here were performed using 1 nM Pif1. (A) Gel image of a representative time course helicase assay with the poly(dT)25/poly(dT)25 fork. (B) Gel image of a representative time course helicase assay with the Ran25/poly(dT)25 fork. (C) Effects of varying the sequence of the loading strand on the unwinding rate.
