TABLE 3.
Evaluation of the total hypoxia/pimonidazole adducts, in A549 cells. Cells were seeded under variable treatments and conditions for different incubation intervals (1, 6, 12, and 24 h). The fluorescence readings of pimonidazole adducts are presented RFU (n = 8). Results are expressed as mean ± standard error.
| Treatment | 1 h | 6 h | 12 h | 24 h |
|---|---|---|---|---|
| Normoxic control | 1023 ± 94 | 1075 ± 76 | 1111 ± 53 | 1162 ± 113 |
| Hypoxic control | 1493 ± 210 **a | 1872 ± 198 **a | 2335 ± 134 ***a | 2630 ± 222 ***a |
| Oxygenated Perftoran (5%)x | 988 ± 62 ***b | 1005 ± 140 ***b | 1034 ± 92 ***b | 1087 ± 82 ***b |
| Carboplatin (20% of IC50)x | 1637 ± 171 *b | 2054 ± 276 *b | 2661 ± 402 *b | 3103 ± 310 *b |
| Perftoran/carboplatin (20% of IC50)x | 1265 ± 156 **c | 1206 ± 193 **c | 1386 ± 122 **c | 1601 ± 167 ***c |
X, under hypoxic conditions; a, compared with normoxic control; b, compared with hypoxic control; c, compared with carboplatin-treated cells, *p< 0.05, **p< 0.01, ***p< 0.001, and ****p< 0.0001.