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The sample prepared with a double-layered media embedding method (A) The solid block (B) The complete sample slice under a microscope (C) MSIs demonstrating the overlay of ions of OCT, 10% gelatin and DAGs in positive mode (D) MSIs exhibiting the overlay of ions of OCT, 10% gelatin and FAs in negative mode. The sample block embedded with 10% gelatin and OCT was frozen in the pre-cooled iso-pentane.
