TABLE 2.
The amounts of various types of components in CCS and NCS samples by TOF-SIMS and qNMR.
| Methods | Chemical components | CCS samples | NCS samples |
|---|---|---|---|
| TOF-SIMS a | total amino acids | 9.16E-04 ± 1.27E-04 | 1.02E-03 ± 1.73E-04 |
| total monosaccharides | 2.17E-03 ± 2.32E-04 | 2.22E-03 ± 3.53E-04 | |
| total nucleosides | 1.40E-03 ± 1.32E-04 | 1.52E-03 ± 1.50E-04 | |
| total fatty acids | 5.20E-03 ± 2.07E-03** | 1.63E-03 ± 2.37E-04 | |
| total glycerides | 2.78E-03 ± 1.16E-03*** | 8.16E-04 ± 2.00E-04 | |
| total MAGs | 6.33E-04 ± 1.78E-04*** | 2.63E-04 ± 6.67E-05 | |
| total DAGs | 2.08E-03 ± 9.69E-04*** | 5.25E-04 ± 1.41E-04 | |
| total TAGs | 6.79E-05 ± 2.04E-05*** | 2.74E-05 ± 4.55E-06 | |
| total glycerophospholipids | 7.39E-05 ± 1.57E-05* | 5.19E-05 ± 1.54E-05 | |
| total PCs | 3.70E-05 ± 8.96E-06 | 2.85E-05 ± 8.60E-06 | |
| total PAs | 1.38E-05 ± 2.93E-06 | 1.11E-05 ± 3.54E-06 | |
| total PIs | 2.32E-05 ± 8.81E-06** | 1.24E-05 ± 4.46E-06 | |
| total sphingolipids | 3.84E-04 ± 1.21E-04 | 3.06E-04 ± 1.57E-04 | |
| total sphingobases | 2.62E-04 ± 1.11E-04 | 2.37E-04 ± 1.36E-04 | |
| total Cers | 2.55E-05 ± 7.27E-06** | 1.51E-05 ± 6.27E-06 | |
| total SMs | 1.88E-05 ± 2.79E-06 | 1.52E-05 ± 1.03E-05 | |
| total Hexcers | 7.76E-05 ± 3.67E-05* | 3.94E-05 ± 1.16E-05 | |
| total sterols | 5.17E-04 ± 9.32E-05 | 5.83E-04 ± 3.26E-04 | |
| total cholesterols | 9.82E-05 ± 2.39E-05 | 1.88E-04 ± 1.78E-04 | |
| total ergosterols | 4.19E-04 ± 7.11E-05 | 3.96E-04 ± 1.68E-04 | |
| Mannitol | 1.54E-04 ± 2.64E-05 | 1.50E-04 ± 2.53E-05 | |
| Vitamin E | 1.17E-05 ± 4.54E-06 | 1.40E-05 ± 5.78E-06 | |
| qNMR b (µmol/g) | total glycerides | 224.10 ± 11.36*** | 93.62 ± 19.59 |
| total TAGs | 138.98 ± 10.81*** | 25.57 ± 11.21 | |
| total DAGs | 59.72 ± 8.34*** | 42.12 ± 6.96 | |
| total MAGs | 25.40 ± 6.11 | 25.93 ± 2.83 |
a
Data were calculated from the normalized peak areas of essential ions by PID.
b
The amount of glycerides in a 1 g sample of C. sinensis was calculated from the calibrated IS, benzoic acid (the signal at δ 7.60 ppm), and normalized as micro moles. *p < 0.05, **p < 0.01, ***p < 0.001 compared with NCS group (n = 9).