Figure 4.

LINC00152 indirectly targets BCL2L2. (A) Gene structure of BCL2L2 showed the target site of miR-16-5p in its 3’-UTR. (B) The luciferase activity was measured using a dual-luciferase reporter assay. (C) A549 cells were transfected with pcDNA3.1 or pcDNA3.1-BCL2L2. Next, the expression of BCL2L2 in A549 cells was tested by reverse transcription-quantitative polymerase chain reaction. (D) A549 cells were treated with NC, LINC00152 siRNA, LINC00152 siRNA + pcDNA3.1 vector or LINC00152 siRNA + BCL2L2 pcDNA3.1 for 0, 12, 24 and 48 h, respectively. Next, Cell Counting kit-8 assay was performed to detect cell viability and (E) flow cytometry was used to measure cell apoptosis. **, P<0.01 compared to control; ^##, P<0.01 compared to LINC00152 siRNA. NC, negative control; OD, optical density; UTR, untranslated region.