TABLE 1.
Bacterial growth, accumulation of pertussis toxin, and viability after treatment
| Treatmenta | Amt of intracellular pertussis toxin (ng/ml)b | OD600c | OD600d
|
Viability (1010 CFU/ml)e
|
||
|---|---|---|---|---|---|---|
| Control | Treated (% of control) | Control | Treated (% of control) | |||
| Erythromycin (10 μg/ml) | 78 ± 16 | 2.77 ± 0.49 | 11.6 ± 1.4 | 4.0 ± 1.2 (35) | 3.0 ± 2.9 | 0.18 ± 0.09 (6) |
| Chloramphenicol (34 μg/ml) | 124 ± 28 | 3.8 ± 0.05 | 12.3 ± 1.11 | 5.2 ± 0.33 (42) | 1.4 ± 1.2 | 0.45 ± 0.3 (32) |
| Rifampin (25 μg/ml) | 239 ± 61 | 3.4 ± 0.58 | 11.7 ± 0.17 | 3.3 ± 0.11 (28) | 5.9 ± 0.1 | 0.48 ± 0.3 (8) |
| Piperacillin (100 μg/ml) | 333 ± 53 | 3.98 ± 0.19 | 13.8 ± 0.46 | 7.5 ± 0.26 (54) | 6.2 ± 0.4 | 0.5 ± 0.2 (8) |
| Cefoperazone (100 μg/ml) | 333 ± 53 | 3.98 ± 0.19 | 13.8 ± 0.46 | 8.7 ± 0.26 (63) | 6.2 ± 0.4 | 0.9 ± 0.1 (14) |
| Polymyxin B (50 μg/ml) | 94 ± 12 | 2.98 ± 0.25 | 12.4 ± 0.59 | 3.3 ± 0.48 (27) | 5.4 ± 0.7 | <0.01 (<0.2) |
| Polymyxin B (25 μg/ml) | 117 ± 30 | 3.6 ± 0.44 | 13.4 ± 0.70 | 3.8 ± 0.47 (28) | 6.3 ± 0.6 | 0.14 ± 0.04 (2) |
| Ethanol (10%) | 158 ± 24 | 3.56 ± 0.31 | 12.2 ± 0.87 | 11.1 ± 1.0 (91) | 5.1 ± 0.9 | 4.1 ± 0.9 (80) |
| Lidocaine (40 mg/ml) | 159 ± 28 | 3.65 ± 0.36 | 11.9 ± 0.23 | 3.2 ± 0.37 (27) | 3.7 ± 0.3 | 0.045 ± 0.02 (1) |
| Procaine (40 mg/ml) | 174 ± 29 | 3.77 ± 0.39 | 12.6 ± 0.69 | 4.7 ± 0.35 (37) | 4.7 ± 1.0 | 0.34 ± 0.05 (7) |
a
Broth cultures were harvested at 24 h. The amount of intracellular pertussis toxin and the OD were determined. The cultures were split into treated (as indicated) and untreated control groups. OD and viability were determined after a 24-h incubation.
b
Amount of intracellular pertussis toxin prior to treatment.
c
OD600 prior to treatment.
d
OD600 after 24 h of the treatment indicated.
e
Viability after 24 h of the treatment indicated.