FIGURE 8.

Dox reversal partially reverses frataxin deficiency in a tissue-specific manner in FRDAkd mice. (A) Western blot analysis in WT ±, TG + , and TG ± mice of frataxin and tissue-specific internal loading controls (actin, SM-actin, GAPDH, and tubulin) in the cerebellum (cere), liver, heart, cortex, and skeletal muscle (SkM) (n = 3 for WT ± , TG +, and TG ±). (B) Quantification of frataxin levels normalized to tissue-specific internal controls (actin, SM-actin, GAPDH, tubulin). Frataxin levels were reported as a percentage of the wild type set to 100 percent. Quantification of SkM was zero in both TG + and TG ± (not shown) (Stats: WT, n = 3, TG +, n = 3, TG ±, n = 3, mean ± SEM, two-tailed, unpaired Student’s t-test, **P < 0.01).