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. 2022 Mar 24;9:848450. doi: 10.3389/fnut.2022.848450

Figure 4.

Figure 4

Medium-chain fatty acid (MCFA) decanoate (C10:0) promotes GLP-1 secretion via GPR84 in STC-1 cells. (A) STC-1 cells were treated with various doses of C10:0 and GPR84 agonist embelin (0.01, 0.03, 0.1, 0.3, 0.6, 1, 2, and 5 mM). GLP-1 concentration in the culture medium was measured (n = 6). Data are represented as relative to the GLP-1 protein levels in non-stimulated cells. (B) RNAi efficiency by Gpr84 siRNA (n = 6). Gpr84 expression is represented as relative to its expression in the untreated control cells. (C) Inhibitory effects of Gpr84 siRNA on GLP-1 secretion in cells treated with C10:0 (1 mM) and embelin (1 mM; n = 4). (D–F) Cells were stimulated with C10:0 (500 μM) after pretreatment with NF023 (10 μM), PTX (1 μg/mL), Gallein (30 μM), U73122 (1 μM), or GW1100 (10 μM) for 20 min (n = 4–6). Data are represented as relative to the GLP-1 protein levels in C10:0 non-stimulated cells. **P < 0.01; *P < 0.05 (Tukey-Kramer test). These experiments were performed using independent cultures from two biological replicates. All data are presented as the mean ± standard error of mean (SEM).