Fig. 3.

Cartilage injury suppresses miR-140-3p and miR-140-5p expression. (A) Safranin O/Fast Green-stained section of 6 week old murine femoral head with schematic showing the femoral head before (intact) and after cartilage injury (avulsed and cut). Femoral heads were either snap frozen immediately in liquid nitrogen to provide the negative control (0h) or cut into 4 pieces and cultured in serum-free media for 4 h at 37°C to measure the biological injury response (B) RNA was extracted and miR-140-3p and miR-140-5p expression were quantified by qPCR, normalised to RNU24 and expressed relative to 0h. Data are shown as mean ± SD. Statistical significance by student two-tailed test. n = 3 biological replicates.