Fig. 4.

Human chondrocyte gene editing by CRISPR-Cas9 identifies novel miR-140 gene targets. Gene expression of 46 genes with putative roles in osteoarthritis or previously described miR-140 targets, were analysed by qPCR on pre-printed TaqMan Microfluidic cards. All genes were normalised to RPLP0 and expressed relative to their respective non-targeting (NT) sgRNA control (for each donor). Genes are displayed according to the strength of the statistical significance from left to right. Gene names marked with “†” are previously identified miR-140 targets. Each color represents an individual donor. Statistical significance was determined using the Bonferroni-Dunn method with corrections for multiple comparison. n = 5 biological replicates (donors). Three genes had P < 0.05 after correction. A further 5 genes whose uncorrected P values were <0.01 are also indicated.