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. 2022 Mar 17;23(5):158. doi: 10.3892/ol.2022.13278

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Copyright: © Feng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — rh-IL-33 and supernatant from cultures primary cancer-associated fibroblasts promote the differentiation of blood-derived monocyte towards M2-like macrophages. (A) Higher expression of M2 markers, including CD209, MRC1, SLC40A1 and FOLR2, in the blood-derived stimulated by supernatant of NOFs and CAFs measured using western blot assays. (B) Higher concentration of IL-10 and TGF-β in the supernatant of NOFs and CAFs after stimulation with IL-33 or the supernatant from primary CAFs. (C) Higher frequency of CD163^high CD209^high M2-macrophages in the blood-derived monocytes stimulated with human recombined IL-33 and supernatant of CAMs. (D) Expression of proinflammatory cytokines including IL-1β, TNF-α and IL-6 in the blood-derived monocytes cultured with human recombined IL-33 and supernatant of CAMs. (E and F) Expressions of NK-κB signaling-related proteins measured using western blot assays. *P<0.05, **P<0.01 and ***P<0.001. SLC40A1, solute carrier family 40 member 1: NOF, normal ovarian fibroblasts; CAF, cancer-associated fibroblasts; rh-IL-33, recombination human interleukin-33; FOLR2, folate receptor β.