Table 1.
Protein:RA KD and N values determined by ITC
| HNF-1A construct | KD (μM) | N (HNF-1A:RA) |
|---|---|---|
| DBD | 0.11 ± 0.05 | 1.89 ± 0.30 |
| DBD/R263C | 2.00 ± 0.14 (p = 0.0076, ∗∗) | 1.74 ± 0.10 |
| DBD/N266S | 0.57 ± 0.05 (p = 0.0008, ∗∗∗) | 1.62 ± 0.18 |
| DD-DBD | 0.10 ± 0.05 | 1.97 ± 0.20 |
| DD-DBD/R263C | 8.00 ± 3.00 (p < 0.0001, ∗∗∗∗) | 1.73 ± 0.14 |
| DD-DBD/N266S | 0.74 ± 0.11 (p = 0.0004, ∗∗∗) | 1.55 ± 0.11 |
Parameters were extracted from binding experiments in which double-stranded RA oligonucleotide was titrated into DBD, DD-DBD, or corresponding HNF-1A variant constructs. p values indicate significant differences of KD values between studied variants and respective WT constructs. (mean ± SD; N = 3)