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. 2022 Mar 24;14:852972. doi: 10.3389/fnagi.2022.852972

FIGURE 1.

FIGURE 1

Neuronal expression of APP and BACE1 leads to an age-dependent amyloid aggregation in the brain. (A) Western blot analysis showing full-length amyloid precursor protein (APP-FL) and C-terminal fragment (APP-CTF) of 10, 30, and 60 DAE flies expressing APP. Stain-Free imaging of a representative portion of the membrane is presented to show the protein loading. (B) Quantification of CTF/FL ratio in each group. Data are expressed as mean ± SD. n = 5/group. One-way ANOVA with Bonferroni’s post hoc test. *P < 0.05, **P < 0.01. (C) Top row: fly brains at 10, 30, and 60 DAE stained with antibody anti APP (heatmap). Bottom row: higher magnification images of the boxed areas in the top row. Scale bars = 30 μm. (D) Scatter plot showing quantification of the number of amyloid plaques in panel (C). The whiskers represent the minimum and maximum values of the dataset. One-way ANOVA with Bonferroni’s post hoc test. n = 7–8/group, ****P < 0.0001.