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. 2022 Mar 24;13:860362. doi: 10.3389/fphar.2022.860362

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Copyright © 2022 Xu, Li, Lin, Liang, Qin, Ding, Chen and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Iso regulates the Nrf2/Keap1 pathway in CS-induced COPD mice. (A) The protein expression levels of Nrf2, Keap1, p62, HO-1, SOD1, and SOD2 were measured by western blotting, respectively. GAPDH was used as the internal reference. (B) The band intensities of Nrf2, Keap1, p62, HO-1, SOD1, and SOD2 were semiquantified using ImageJ (n = 3 mice per group). Data was shown as mean (±SD) (B). To test for group differencses one-way ANOVA (B) was applied. ^## p < 0.01, ^### p < 0.001 vs. control group, *p < 0.05, **p < 0.01, ***p < 0.001 vs. CS group and ⁺⁺ p < 0.01, ⁺⁺⁺ p < 0.001 vs. CS + Iso 30 mg/kg group.