Figure 1.

Re-expression of RelA renders tumor cells sensitive to Ref-1 inhibitors. KC3590 cells that underwent functional deletion of RelA (Parent and Vector) and KC3590 clone lines that express functional RelA (C13, C15) were challenged with different concentrations of APX3330 (A), APX2009 (B) and APX2014 (C), RN7-58 (D), ARi3 (E) for 48 hours. Cytotoxicity was measured by Alamar blue (4 hr incubation) at 590 nm. Error bars represent standard error (N = 3). Statistical differences between EC₅₀s of Ref-1 inhibitors in KC3590 cells are provided in Table 1 .