Figure 2.

Ref-1 inhibitors suppress inflammatory responses via RelA mediated pathways. KC3590 cells that are RelA deficient (Vector) and clone with functional RelA add back (C13) were challenged with Ref-1 inhibitors, APX3330 (60 μM Vector; 30 μM C13), APX2009 (16 μM Vector; 6 μM C13) based on their EC50 for 24 hours. The effects of Ref-1 inhibitors on mRNA expression levels of IL-8, FOSB, c-Jun was assessed with qPCR (Figure (A–C), respectively). Six independent experiments were performed (n = 6). Hashtag “#” is comparing to C13 DMSO, ^#p < 0.05, ^###p < 0.0001; ^*p < 0.05, ^**p < 0.01, ^***p < 0.0001, ANCOVA, N=6. “ns”, not significant.