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. 2022 Mar 24;13:787146. doi: 10.3389/fmicb.2022.787146

TABLE 1.

Physicochemical soil parameters were measured at the sampling site and included radiocarbon dating (14C), which was measured only in 2019 and was calculated in fM (*) for samples younger than 1963 or BP (**) for samples older than 1945.

Depth [cm] 14C [*fM **BP] H2O [%] SOM [%] pH Horizon Layer


2019 2020 2019 2020 2019 2020 2019 2020
0–10 *1.04 7.94 ± 1.03 28.80 ± 3.38 16.01 ± 4.22 8.73 ± 1.82 3.87 ± 0 4.22 ± 0.03 O1 AL

10–20
*1.13 11.77 ± 0.93 22.52 ± 0.73 7.09 ± 1.71 5.39 ± 0.83 4.46 ± 0.05 4.02 ± 0.05 M1
20–30
*1.16 11.83 ± 0.34 22.05 ± 3.30 5.75 ± 0.21 10.19 ± 3.22 4.25 ± 0.02 4.29 ± 0.01
30–40 *1.20 13.13 ± 3.12 26.57 ± 0.83 5.85 ± 2.23 13.68 ± 0.44 4.73 ± 0.09 4.25 ± 0.01

40–50
**2,635 28.27 ± 4.92 7.73 ± 1.58 24.42 ± 4.78 2.59 ± 0.68 5.08 ± 0.05 4.63 ± 0.03 O2 TZ TZ
50–60 **3,770 23.49 ± 1.92 15.61 ± 6.28 15.76 ± 4.50 2.93 ± 0.30 5.45 ± 0.10 4.13 ± 0.02

60–70 **26,500 14.14 ± 8.43 8.72 ± 2.53 3.78 ± 0.09 1.52 ± 0.16 6.54 ± 0.05 4.86 ± 0.05 M2
70–80
**22,100 16.31 ± 1.09 6.35 ± 0.60 3.99 ± 0.25 1.10 ± 0.24 7.01 ± 0.01 4.48 ± 0 PF
80–90
**26,200 1.65 ± 4.43 7.96 ± 0.61 1.78 ± 0.30 1.00 ± 0.20 6.61 ± 0 4.57 ± 0.01
90–100 NA NA 7.80 ± 0.59 NA 1.04 ± 0.26 NA 4.91 ± 0.01 PF

In both years, weight-based relative soil moisture (H2O) and soil organic matter (SOM) were measured based on loss on ignition and the pH was determined in triplicates and standard deviation is indicated (±). Layers included the active layer (AL, 0-40 cm) as long-term seasonally thawing material above the former ice lens, the transition zone (TZ, 2019: 40-70 cm, 2020: 40-90 cm) as the newly thawed material since the collapse, and permafrost (PF, 2019: > 70 cm, 2020: > 90 cm) as the still frozen ground. Based on SOM and 14C, four separate horizons were visible, namely the recent surface organic horizon (O1, 0-10 cm), the recent underlying mineral horizon (M1, 10-40 cm), the older buried organic horizon (O2, 40-60 cm), and an ancient mineral horizon (M2, > 60 cm).