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. 2022 Mar 24;13:861670. doi: 10.3389/fimmu.2022.861670

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Copyright © 2022 Gray, Biron-Girard, Wakeley, Chung, Chen, Quiles-Ramirez, Tolbert and Ayala

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adoptive transfer of Jurkat T_regs improves survival of VISTA^-/- mice post CLP. Blockade of VISTA in Jurkat T_regs in vitro reduces viability and cytokine production. (A) 14-day survival following adoptive transfer and CLP [VISTA^-/-(HBSS alone): n = 11, VISTA^-/-(HBSS+ Jurkat T_regs): n = 10]. (B) Alamar Blue viability assay of Jurkat T_regs following treatment with Ig control or 13F3. (C–I) Supernatant cytokine concentration of Jurkat T_regs following treatment with plasma from septic mouse (stimulated) or plasma and 13F3 (stim. + 13F3). Summary graphs show mean ± SEM; not significant ^ns; significance ^#p = 0.05; *p < 0.05; **p < 0.01.