Figure 2. C6G25S targeted and inhibited various strains of SARS‐CoV‐2.

1. C6 targets a highly conserved region of the virus RdRp (accession number: NC_045512.2). The figure shows a genome map for five variants of concern (VOC), two variants of interests (VOI), and five other variants. Dots above the genome indicate the locations of typical mutations for each variant. Important amino acid mutations listed in the Spike mutations of interest on the European Centre for Disease Prevention and Control (ECDC) website are labeled in red. Other mutations are labeled in black. The target site and sequence for C6G25 recognition on RdRp is shown below the map. C6G25 sequence is shown in red and the viral sequence in black.
2. IC₅₀ for C6G25S against different variants. Vero E6 cells were transfected with 10, 2, 0.4, 0.08, or 0.016 nM of C6G25S before infection with different strains of SARS‐CoV‐2. The viral RNA was quantitated by RT‐qPCR at 24 h after infection. Data are presented as mean ± SD of three biological replicates.

Source data are available online for this figure.