Figure EV4. No significant immune stimulation was observed in mice treated with high‐dose of C6G25S.

1. ICR mice (n = 3) treated with saline control or different dosages (20, 40, 75 mg/kg) of C6G25S by intranasal instillation. The bronchoalveolar lavage (BAL) was collected at day 2 after treatment. Different WBC cell counts were analyzed by a hematology analyzer. The cell counts are expressed as mean ± SD. All of the different treatment groups are statistically insignificant compared with the control in Student’s t‐test.
2. Lung tissues of each mouse were collected, and RNA was extracted. The mRNA expression of IL‐6, TNF‐α, IFN‐α, and IFN‐γ was quantitated by RT‐PCR. The relative amount of each cytokine is presented as mean ± SD. All of the different treatment groups are statistically insignificant compared with the control in Student’s t‐test.