Atherosclerosis experiment design in Fmr1+/+ and Fmr1−/− mice that were injected with AAV_PCSK9 and fed WD (16 weeks).
Lesion area calculated from en face aorta, stained with ORO (n = 12–13 mice per group; Scale bar = 5 mm).
Total plaque area was calculated from hematoxylin & eosin (H&E)‐stained aortic root sections (n = 8 mice per group; Scale bar = 300 µm).
Foam cell area was calculated from ORO‐stained aortic root sections (n = 8 mice per group; Scale bar = 300 µm).
Necrotic area was calculated from H&E‐stained aortic root sections (n = 8 mice per group; Scale bar = 100 µm).
Atherosclerosis experiment design in myFmr1+/+ and myFmr1−/− mice that were injected with AAV_PCSK9 and fed WD (16 weeks).
Lesion area calculated from en face aorta, stained with ORO (n = 9 mice per group; Scale bar = 5 mm).
Total plaque area was calculated from H&E‐stained aortic root sections (n = 9–6 mice per group; Scale bar = 300 µm).
Foam cell area was calculated from ORO‐stained aortic root sections (n = 9–6 mice per group; Scale bar = 300 µm).
Necrotic area was calculated from H&E‐stained aortic root sections (n = 9–6 mice per group; Scale bar = 100 µm).