Figure 5.

Quantification of mammary organoid size and morphology under different culture conditions. Quantification of mammary organoid area (A), organoid length (B), and organoid core size (C). (D) Organoid length/core area was determined as a robust parameter to accurately describe the difference between complex branched organoids and hyperbranched or budding organoids. All parameters were quantified at day 18 of culture for all conditions; data represent >30 organoids per condition derived from three experimental replicates. Significance was tested using a Mann–Whitney test, ^**p ≤ 0.01, and ^****p ≤ 0.0001.