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. 2022 Mar 16;13:826107. doi: 10.3389/fphys.2022.826107
Total volume of neutralized collagen (μl) 500 1,000
Collagen I stock (μl) 434 868
10X DMEM low glucose (μl) 50 100
0.5 N NaOH (μl) 2.5 5