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. 2022 Mar 24;13:803121. doi: 10.3389/fmicb.2022.803121

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Copyright © 2022 Aguilera, Mascardi, Belforte, Rosso, Quesada, Llovet, Iraola, Trinks and Penas-Steinhardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Volcano Plot of PICRUSt analysis. Significant metabolic pathways down-regulated in the gut microbiota of individuals during the PCSI are represented in different colors with their corresponding MetaCyc ID. Metabolic pathways without a statistically significant difference between groups are shown in gray. The W-value represents the number of times the null-hypothesis (the average abundance of a given feature in a group is equal to that in the other group) was rejected for a given metabolic pathway. Red dash lines indicate a significance a priori threshold for differentially abundance set at W ≥ 324 (W > 80% of the total number of metabolic pathways) and clr (centered logarithmic ratio) > |2|.