Figure 3.

Scoring pipeline performance on a multi-day fear conditioning and extinction protocol. (A) Average time classified SWS, REM, quiet wakefulness, and freezing during the rest sessions before exposure to the testing environments for each day of the behavioral protocol. (B) Same as (A) but for the first 3 min in the conditioning context A. (C) Same as (A) but for the last 3 min in the conditioning context A. (D) Same as (A) but for rest sessions following exposure to the testing environments. (E) Average time spent sleeping across auditory cued extinction training sessions in context B for all animals; red dashed vertical lines: CS+ onsets; ochre dashed vertical lines: CS− onsets. (F) State scoring in a representative session of late extinction of cued fear involving both sleep and freezing (see Supplementary Video 5). (Top) Animal speed as measured by head movements; red dashed vertical lines: CS+ onsets; ochre dashed vertical lines: CS− onsets. Time stamps of CSs presentations corresponding to those overlaid in Supplementary Video 5 frames are indicated below the plots. Shaded area: periods classified as freezing (red), quiet wakefulness (green), SWS (blue), and REM (yellow). Note how the animal did not freeze during CS− presentations, but the onsets of the first three CS+ triggered freezing responses. (Bottom) Spectrogram of mPFC LFP activity; white squares: intervals of elevated sleep spindle power scored as SWS. The rat started to sleep after the fourth CS+ presentation and from then on, every CS+ presentation woke him up, but he fell asleep again within 30–40 s.