Table 2.
Characteristic distribution of TILs in CCA
| Ref | Author | Year | Country | Sample(n) | Location of TILs |
Subtypes of TILs | Assessment of TILs | Distribution (number or density of TILs) |
|---|---|---|---|---|---|---|---|---|
| [31] | Zhou G | 2019 | China | CCA (26) | IT vs. PT | CD8+ / FoxP3+ / CD4+ / CD56+ | IHC | CD8+, CD4+: PT > IT; Foxp3+, CD56: no difference |
| [17] | Asahi Y | 2020 | Japan | iCCA (78) | IT vs. PT | CD8+ / FoxP3+ | IHC |
CD8+: PT > IT (91.0±89.9 vs. 41.1±54.1) Foxp3+: PT > IT (18.9±21.5 vs. 11.5±15.7) |
| [23] | Lu JC | 2019 | China | iCCA (320) | IT vs. PT | PD1(+)T | IHC | PT < IT (40±5 VS 60.1±6.5; p< 0.01) |
| [32] | Kasper HU | 2009 | China | CCA (27) | IT vs. PT | CD3+ / CD4+ / CD8+/CD20 | IHC |
PT > IT CD3: 52.6±28.5 vs 310.4± 202.0, p=0.008 CD4: 18.0±22.3 vs 223.1±43.2, p=0.043 CD8: 40.7± 30.5 vs 118.7± 35.5, p≤ 0.001 CD20: 11.1 (± 11.8) vs 0.1 (± 0.3), P = 0.035 |
| [27] | Ueno T | 2018 | Japan | eCCA (117) | IT vs. PT | CD4+ / CD8+ / FoxP3+ | IHC |
No difference CD4+(median 77 vs 59, p=0.16) CD8+(median 52 vs 55, p=0.94) Foxp3+(median 9 vs 9, p=0.62) |
| [18] | Goeppert B | 2013 | Germany | eCCA (149) iCCA (157) GBAC (69) | IT vs. PT | CD4+ / CD8+ / FoxP3+/CD20 | IHC |
CD4+: PT > IT CD8+: PT > IT CD20: No foud Foxp3+: PT < IT |
| [30] | Xu YP | 2021 | China | iCCA (140) | IT vs. PT | CD8+ | IHC | PT > IT |
| [26] | Tian L | 2020 | China | iCCA (322) | IT vs. PT | CD8+ | mIHC | PT > IT (p<0.001) |
| [29] | Wu H | 2021 | China | iCCA (50) | IT vs. PT | CD8+ / CD3+ | IHC | PT > IT (p=0.009, p=0.047) |
| [20] | Kim HD | 2021 | Korea | CCA (52) | IT vs. PT | CD8+ / CD4+ / FoxP3+ | mIHC | PT > IT (p<0.001, p<0.001, p<0.001) |
| [33] | Kim HD | 2021 | Korea | iCCA (33) | IT vs. PT | CD103+CD8+ | mIHC | PT < IT (mean 1.4/mm2 vs 1.8/mm2) |
Various studies investigated the spatial distribution of tumor-infiltrating lymphocytes in cholangiocarcinoma
CD, cluster of differentiation, CCA cholangiocarcinoma, eCCA extrahepatic cholangiocarcinoma, FOXP3 forkhead box P3, GBAC gallbladder cancer, iCCA intrahepatic cholangiocarcinoma, IHC Immunohistochemistry, IT intratumoral, mIHC multiplexed immunohistochemistry, PD programmed cell death protein, PT peritumoral, Ref reference, Th helper T cell, TIL tumor-infiltrating lymphocytes